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Crystal structure of Meaban virus nucleoside-2'-O-methyltransferase

X-ray diffraction
2.9Å resolution
PDB entry 2oxt coloured by chain, front view.
PDB entry 2oxt coloured by chain, side view.
PDB entry 2oxt coloured by chain, top view.
Source organism: Meaban virus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: NUCLEOSIDE-2'-O-METHYLTRANSFERASE
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Multimeric state: monomeric
Accessible surface area: 12599.73 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106067
Assembly 2 (preferred)
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Assembly Name: NUCLEOSIDE-2'-O-METHYLTRANSFERASE
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Multimeric state: monomeric
Accessible surface area: 12551.14 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106067
Assembly 3
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Assembly Name: NUCLEOSIDE-2'-O-METHYLTRANSFERASE
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Multimeric state: monomeric
Accessible surface area: 12894.41 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106067
Assembly 4
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Assembly Name: NUCLEOSIDE-2'-O-METHYLTRANSFERASE
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Multimeric state: monomeric
Accessible surface area: 12537.32 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106067

Macromolecules

NUCLEOSIDE-2'-O-METHYLTRANSFERASE
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 29.65 KDa
Source organism: Meaban virus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0EKU1 (Residues: 2516-2780; Coverage: 8%)
Pfam: FtsJ-like methyltransferase
InterPro:
CATH: Vaccinia Virus protein VP39
NUCLEOSIDE-2'-O-METHYLTRANSFERASE in PDB entry 2oxt, assembly 2, front view.
NUCLEOSIDE-2'-O-METHYLTRANSFERASE in PDB entry 2oxt, assembly 2, side view.
NUCLEOSIDE-2'-O-METHYLTRANSFERASE in PDB entry 2oxt, assembly 2, top view.
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