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Solution structure of PICK1 PDZ in complex with the carboxyl tail peptide of GluR2

Solution NMR
PDB entry 2pku coloured by chain, ensemble of 20 models, front view.
PDB entry 2pku coloured by chain, ensemble of 20 models, side view.
PDB entry 2pku coloured by chain, ensemble of 20 models, top view.
Source organism: Rattus norvegicus
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: PRKCA-binding protein and peptide
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Multimeric state: hetero dimer
Accessible surface area: 5261.6 Å2
Buried surface area: 812.36 Å2
Dissociation area: 406.18 Å2
Dissociation energy (ΔGdiss): 0.96 kcal/mol
Dissociation entropy (TΔSdiss): 5.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-552687

Macromolecules

PRKCA-binding protein
Chain: A
Length: 87 amino acids
Theoretical weight: 9.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EP80 (Residues: 18-104; Coverage: 21%)
Gene names: Pick1, Prkcabp
Pfam: PDZ domain
InterPro:
CATH: Pdz3 Domain
PRKCA-binding protein in PDB entry 2pku, assembly 1, front view.
PRKCA-binding protein in PDB entry 2pku, assembly 1, side view.
PRKCA-binding protein in PDB entry 2pku, assembly 1, top view.
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peptide (GLU)(SER)(VAL)(LYS)(ILE)
Chain: B
Length: 5 amino acids
Theoretical weight: 576 Da
Source organism: Rattus norvegicus
Expression system: Not provided
peptide (GLU)(SER)(VAL)(LYS)(ILE) in PDB entry 2pku, assembly 1, front view.
peptide (GLU)(SER)(VAL)(LYS)(ILE) in PDB entry 2pku, assembly 1, side view.
peptide (GLU)(SER)(VAL)(LYS)(ILE) in PDB entry 2pku, assembly 1, top view.
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