› 2q1k

X-ray diffraction
2.7Å resolution

Cyrstal Structure of AscE from Aeromonas hydrophilla

Released:
DOI: 10.2210/pdb2q1k/pdb
PDB entry 2q1k coloured by chain, front view.
PDB entry 2q1k coloured by chain, side view.
PDB entry 2q1k coloured by chain, top view.
Source organism: Aeromonas hydrophila
Primary publication:
Structure of AscE and induced burial regions in AscE and AscG upon formation of the chaperone needle-subunit complex of type III secretion system in Aeromonas hydrophila.
Tan YW, Yu HB, Leung KY, Sivaraman J, Mok YK
Protein Sci 17 1748-60 (2008)
PMID: 18662905

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AscE Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 67 amino acids
Theoretical weight: 7.8 KDa
Source organism: Aeromonas hydrophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1EHA4 (Residues: 1-67; Coverage: 100%)
Gene name: ascE
Sequence domains: Type III secretion system, cytoplasmic E component of needle
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 69.039Å b: 69.039Å c: 105.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.288
Expression system: Escherichia coli

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Citations

1 review citation

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity.
Fernández-Bravo et al. (2020)
8 other articles

2 mentions without citation

Crystal structure of the heteromolecular chaperone, AscE-AscG, from the type III secretion system in Aeromonas hydrophila.
Chatterjee et al. (2011)
1 more