Structure analysis

Cyrstal Structure of AscE from Aeromonas hydrophilla

X-ray diffraction
2.7Å resolution
Source organism: Aeromonas hydrophila
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Assembly Name: AscE
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Multimeric state: homo dimer
Accessible surface area: 6443.63 Å2
Buried surface area: 1991.16 Å2
Dissociation area: 995.58 Å2
Dissociation energy (ΔGdiss): 8.73 kcal/mol
Dissociation entropy (TΔSdiss): 10.18 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-172817
Assembly 2
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Assembly Name: AscE
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Multimeric state: homo dimer
Accessible surface area: 6417.32 Å2
Buried surface area: 2171.97 Å2
Dissociation area: 1,085.98 Å2
Dissociation energy (ΔGdiss): 7.86 kcal/mol
Dissociation entropy (TΔSdiss): 10.24 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-172817

Macromolecules

Chains: A, B, C, D
Length: 67 amino acids
Theoretical weight: 7.8 KDa
Source organism: Aeromonas hydrophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1EHA4 (Residues: 1-67; Coverage: 100%)
Gene name: ascE
Pfam: Type III secretion system, cytoplasmic E component of needle
InterPro: Type III export protein PscE/YscE
CATH: Immunoglobulin FC, subunit C

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