› 2q34

X-ray diffraction
1.85Å resolution

Crystal Structure of the ECH2 decarboxylase domain of CurF from Lyngbya majuscula, rhombohedral crystal form

Released:
DOI: 10.2210/pdb2q34/pdb
PDB entry 2q34 coloured by chain, front view.
PDB entry 2q34 coloured by chain, side view.
PDB entry 2q34 coloured by chain, top view.
Source organism: Moorena producens 19L
Primary publication:
Crystal structure of the ECH2 catalytic domain of CurF from Lyngbya majuscula. Insights into a decarboxylase involved in polyketide chain beta-branching.
Geders TW, Gu L, Mowers JC, Liu H, Gerwick WH, Håkansson K, Sherman DH, Smith JL
J Biol Chem 282 35954-63 (2007)
PMID: 17928301

Function and Biology Details

Reaction catalysed: 
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CurF Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.1 KDa
Source organism: Moorena producens 19L
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DNE7 (Residues: 17-257; Coverage: 8%)
Gene name: curF
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R32
Unit cell:
a: 106.241Å b: 106.241Å c: 118.496Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.206
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Isoprenoid-like alkylations in polyketide biosynthesis.
Calderone CT. (2008)
7 more

69 mentions without citation

Neuregulin 1 in neural development, synaptic plasticity and schizophrenia.
Mei et al. (2008)
68 more