› 2qf4

X-ray diffraction
1.2Å resolution

High resolution structure of the major periplasmic domain from the cell shape-determining filament MreC (orthorhombic form)

Released:
DOI: 10.2210/pdb2qf4/pdb
PDB entry 2qf4 coloured by chain, front view.
PDB entry 2qf4 coloured by chain, side view.
PDB entry 2qf4 coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
High-resolution structure of the major periplasmic domain from the cell shape-determining filament MreC.
Lovering AL, Strynadka NCJ
J Mol Biol 372 1034-1044 (2007)
PMID: 17707860

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell shape-determining protein MreC Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 18.23 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8DMY2 (Residues: 106-272; Coverage: 61%)
Gene names: mreC, spr2023
Sequence domains: rod shape-determining protein MreC
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 50.247Å b: 70.362Å c: 89.763Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.167
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Structural perspective of peptidoglycan biosynthesis and assembly.
Lovering et al. (2012)
2 more