Structure analysis

Structural basis for the regulation of N-acetylglutamate kinase by PII in Arabidopsis thaliana

X-ray diffraction
2.51Å resolution
Source organism: Arabidopsis thaliana
Assembly composition:
hetero dodecamer (preferred)
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dodecamer
Accessible surface area: 83408.57 Å2
Buried surface area: 52951.8 Å2
Dissociation area: 3,457.07 Å2
Dissociation energy (ΔGdiss): 9.92 kcal/mol
Dissociation entropy (TΔSdiss): 28.81 kcal/mol
Symmetry number: 6
PDBe Complex ID: PDB-CPX-193441

Macromolecules

Chains: A, B
Length: 298 amino acids
Theoretical weight: 31.25 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SCL7 (Residues: 51-347; Coverage: 86%)
Gene names: At3g57560, NAGK, T8H10.160
Pfam: Amino acid kinase family
InterPro:
CATH: Acetylglutamate kinase-like

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Chains: C, D
Length: 135 amino acids
Theoretical weight: 14.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZST4 (Residues: 63-196; Coverage: 68%)
Gene names: At4g01900, GLB1, GLNB1, T7B11.16
Pfam: Nitrogen regulatory protein P-II
InterPro:
CATH: Alpha-Beta Plaits

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