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Crystal structure of Salmonella typhimurium SurE at 2.75 angstrom resolution in monoclinic form

X-ray diffraction
2.71Å resolution
PDB entry 2v4o coloured by chain, front view.
PDB entry 2v4o coloured by chain, side view.
PDB entry 2v4o coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: 5'/3'-nucleotidase SurE
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Multimeric state: homo dimer
Accessible surface area: 20653.71 Å2
Buried surface area: 7827.31 Å2
Dissociation area: 3,690.13 Å2
Dissociation energy (ΔGdiss): 58.67 kcal/mol
Dissociation entropy (TΔSdiss): 14.15 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-159190
Assembly 2 (preferred)
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Assembly Name: 5'/3'-nucleotidase SurE
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Multimeric state: homo dimer
Accessible surface area: 21065.4 Å2
Buried surface area: 8248.39 Å2
Dissociation area: 3,775.74 Å2
Dissociation energy (ΔGdiss): 62.92 kcal/mol
Dissociation entropy (TΔSdiss): 14.19 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-159190

Macromolecules

5'/3'-nucleotidase SurE
Chains: A, B, C, D
Length: 267 amino acids
Theoretical weight: 28.62 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P66881 (Residues: 1-253; Coverage: 100%)
Gene names: STM2927, surE
Pfam: Survival protein SurE
InterPro:
CATH: Survival protein SurE-like phosphatase/nucleotidase
5'/3'-nucleotidase SurE in PDB entry 2v4o, assembly 2, front view.
5'/3'-nucleotidase SurE in PDB entry 2v4o, assembly 2, side view.
5'/3'-nucleotidase SurE in PDB entry 2v4o, assembly 2, top view.
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