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F86A mutant of E9 DNase domain in complex with Im9

X-ray diffraction
1.6Å resolution
PDB entry 2vlq coloured by chain, front view.
PDB entry 2vlq coloured by chain, side view.
PDB entry 2vlq coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Colicin-E9 and Colicin-E9 immunity protein
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Multimeric state: hetero dimer
Accessible surface area: 11226.99 Å2
Buried surface area: 2263.05 Å2
Dissociation area: 741.11 Å2
Dissociation energy (ΔGdiss): 1.24 kcal/mol
Dissociation entropy (TΔSdiss): 10.67 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140796

Macromolecules

Colicin-E9 immunity protein
Chain: A
Length: 86 amino acids
Theoretical weight: 9.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Pfam: Colicin immunity protein / pyocin immunity protein
InterPro:
CATH: Colicin E immunity protein
SCOP: Colicin E immunity proteins
Colicin-E9 immunity protein in PDB entry 2vlq, assembly 1, front view.
Colicin-E9 immunity protein in PDB entry 2vlq, assembly 1, side view.
Colicin-E9 immunity protein in PDB entry 2vlq, assembly 1, top view.
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Colicin-E9
Chain: B
Length: 134 amino acids
Theoretical weight: 15.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Pfam: Colicin/Pyocin-S2, DNase domain
InterPro:
CATH: Colicin/pyocin, DNase domain
Colicin-E9 in PDB entry 2vlq, assembly 1, front view.
Colicin-E9 in PDB entry 2vlq, assembly 1, side view.
Colicin-E9 in PDB entry 2vlq, assembly 1, top view.
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