› 2vs0

X-ray diffraction
1.4Å resolution

Structural analysis of homodimeric staphylococcal aureus virulence factor EsxA

Released:
DOI: 10.2210/pdb2vs0/pdb
PDB entry 2vs0 coloured by chain, front view.
PDB entry 2vs0 coloured by chain, side view.
PDB entry 2vs0 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure of Staphylococcus aureus EsxA suggests a contribution to virulence by action as a transport chaperone and/or adaptor protein.
Sundaramoorthy R, Fyfe PK, Hunter WN
J Mol Biol 383 603-14 (2008)
PMID: 18773907

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-573201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type VII secretion system extracellular protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 11.19 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99WU4 (Residues: 1-97; Coverage: 100%)
Gene names: SAV0282, esxA
Sequence domains: Proteins of 100 residues with WXG
Structure domains: ESAT-6-like

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand CAC 1 x CAC
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 25.773Å b: 39.069Å c: 43.029Å
α: 67.41° β: 84.51° γ: 88.76°
R-values:
R R work R free
0.169 0.166 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

ESX secretion systems: mycobacterial evolution to counter host immunity.
Gröschel et al. (2016)
9 more

5 mentions without citation

Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for the EsxW Beijing variant in Vietnam.
Holt et al. (2018)
4 more