› 2w3e

X-ray diffraction
1.6Å resolution

Oxidized structure of the first GAF domain of Mycobacterium tuberculosis DosS

Released:
DOI: 10.2210/pdb2w3e/pdb
PDB entry 2w3e coloured by chain, front view.
PDB entry 2w3e coloured by chain, side view.
PDB entry 2w3e coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structural insight into the heme-based redox sensing by DosS from Mycobacterium tuberculosis.
Cho HY, Cho HJ, Kim YM, Oh JI, Kang BS
J Biol Chem 284 13057-67 (2009)
PMID: 19276084

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxygen sensor histidine kinase response regulator DevS/DosS Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 16.76 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WGK3 (Residues: 63-210; Coverage: 26%)
Gene names: Rv3132c, devS, dosS
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 36Å b: 86.865Å c: 100.642Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.234
Expression system: Escherichia coli BL21

Quick links

Citations

24 review citations

Virulence factors of the Mycobacterium tuberculosis complex.
Forrellad et al. (2013)
23 more

6 mentions without citation

Insights into redox sensing metalloproteins in Mycobacterium tuberculosis.
Chim et al. (2014)
5 more