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Structure A of CRISPR endoribonuclease Cse3 bound to 19 nt RNA

X-ray diffraction
2.5Å resolution
PDB entry 2y9h coloured by chain, front view.
PDB entry 2y9h coloured by chain, side view.
PDB entry 2y9h coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 13887.53 Å2
Buried surface area: 2229.88 Å2
Dissociation area: 1,114.94 Å2
Dissociation energy (ΔGdiss): 22.17 kcal/mol
Dissociation entropy (TΔSdiss): 10.73 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 2
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 13686.62 Å2
Buried surface area: 2191.65 Å2
Dissociation area: 1,095.83 Å2
Dissociation energy (ΔGdiss): 20.25 kcal/mol
Dissociation entropy (TΔSdiss): 10.69 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 3
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 13881.82 Å2
Buried surface area: 2132.55 Å2
Dissociation area: 1,066.27 Å2
Dissociation energy (ΔGdiss): 22.42 kcal/mol
Dissociation entropy (TΔSdiss): 10.71 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 4
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 12736.25 Å2
Buried surface area: 2107.77 Å2
Dissociation area: 1,053.88 Å2
Dissociation energy (ΔGdiss): 21.38 kcal/mol
Dissociation entropy (TΔSdiss): 10.64 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 5
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 12727.34 Å2
Buried surface area: 2009.6 Å2
Dissociation area: 1,004.8 Å2
Dissociation energy (ΔGdiss): 16.59 kcal/mol
Dissociation entropy (TΔSdiss): 10.64 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 6
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 12560.22 Å2
Buried surface area: 1853.2 Å2
Dissociation area: 926.6 Å2
Dissociation energy (ΔGdiss): 14.7 kcal/mol
Dissociation entropy (TΔSdiss): 10.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 7
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 12235.91 Å2
Buried surface area: 1949.89 Å2
Dissociation area: 974.94 Å2
Dissociation energy (ΔGdiss): 17.31 kcal/mol
Dissociation entropy (TΔSdiss): 10.58 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181
Assembly 8
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Assembly Name: CRISPR-associated endoribonuclease Cse3 and RNA
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Multimeric state: hetero dimer
Accessible surface area: 13207.53 Å2
Buried surface area: 857.42 Å2
Dissociation area: 428.71 Å2
Dissociation energy (ΔGdiss): 2.37 kcal/mol
Dissociation entropy (TΔSdiss): 10.23 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176181

Macromolecules

CRISPR-associated endoribonuclease Cse3
Chains: A, C, E, G, I, K, M, O
Length: 215 amino acids
Theoretical weight: 24.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Pfam: CRISPR associated protein
InterPro: CRISPR-associated protein Cse3
CATH:
CRISPR-associated endoribonuclease Cse3 in PDB entry 2y9h, assembly 1, front view.
CRISPR-associated endoribonuclease Cse3 in PDB entry 2y9h, assembly 1, side view.
CRISPR-associated endoribonuclease Cse3 in PDB entry 2y9h, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q53WG9  
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RNA chains B, D, F, H, J, L, N, P
Name: 5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*UP)-3'
Representative chains: B, D, F, H, J, L, N, P
Source organism: Thermus thermophilus HB8 [300852]
Expression system: Not provided
Length: 19 nucleotides
Theoretical weight: 6.07 KDa
5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*UP)-3' in PDB entry 2y9h, assembly 1, front view.
5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*UP)-3' in PDB entry 2y9h, assembly 1, side view.
5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*UP)-3' in PDB entry 2y9h, assembly 1, top view.
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