› 2yh0

Solution NMR

Solution structure of the closed conformation of human U2AF65 tandem RRM1 and RRM2 domains

Released:
DOI: 10.2210/pdb2yh0/pdb
PDB entry 2yh0 coloured by chain, ensemble of 10 models, front view.
PDB entry 2yh0 coloured by chain, ensemble of 10 models, side view.
PDB entry 2yh0 coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF.
Mackereth CD, Madl T, Bonnal S, Simon B, Zanier K, Gasch A, Rybin V, Valcárcel J, Sattler M
Nature 475 408-11 (2011)
PMID: 21753750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 21.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 148-342; Coverage: 41%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 66%
Refinement method: MODIFIED ARIA
Expression system: Escherichia coli BL21

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Citations

28 review citations

Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell.
Wei et al. (2016)
27 more

11 mentions without citation

Investigating the Role of Large-Scale Domain Dynamics in Protein-Protein Interactions.
Delaforge et al. (2016)
10 more