› 2ze7

X-ray diffraction
2.1Å resolution

Crystal Structure of adenosine phosphate-isopentenyltransferase complexed with zinc ion and substrate analog, DMASPP

Released:
DOI: 10.2210/pdb2ze7/pdb
PDB entry 2ze7 coloured by chain, front view.
PDB entry 2ze7 coloured by chain, side view.
PDB entry 2ze7 coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis.
Sugawara H, Ueda N, Kojima M, Makita N, Yamaya T, Sakakibara H
Proc Natl Acad Sci U S A 105 2734-9 (2008)
PMID: 18258747

Function and Biology Details

Reaction catalysed: 
Prenyl diphosphate + AMP = diphosphate + N(6)-prenyladenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate dimethylallyltransferase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.84 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58758 (Residues: 1-243; Coverage: 100%)
Gene names: AGR_pTi_290, Atu6164, tzs
Sequence domains: Isopentenyl transferase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand DST 1 x DST
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45PX
Spacegroup: P42212
Unit cell:
a: 97.933Å b: 97.933Å c: 65.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.254
Expression system: Escherichia coli

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Citations

6 review citations

Cytokinin action in plant development.
Werner et al. (2009)
5 more

1 mention without citation

Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis.
Sugawara et al. (2008)