› 3tu4

X-ray diffraction
3Å resolution

Crystal structure of the Sir3 BAH domain in complex with a nucleosome core particle.

Released:
DOI: 10.2210/pdb3tu4/pdb
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PDB entry 3tu4 coloured by chain, front view.
PDB entry 3tu4 coloured by chain, side view.
PDB entry 3tu4 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.
Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE
Science 334 977-82 (2011)
PMID: 22096199

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer (preferred)
hetero decamer
monomeric
PDBe Complex ID:
PDB-CPX-116944 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 135 amino acids
Theoretical weight: 15.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
Histone H2A type 1 Chains: C, G
Histone H2B 1.1 Chains: D, H
Regulatory protein SIR3 Chains: K, L
DNA (146-MER) Chain: I
DNA (146-MER) Chain: J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P61
Unit cell:
a: 102.88Å b: 102.88Å c: 555.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.241
Expression system: Escherichia coli

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Citations

42 review citations

New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?
Luger et al. (2012)
41 more

28 mentions without citation

Nucleosome structure and function.
McGinty et al. (2015)
27 more

PDB-REDO

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