› 3a37

X-ray diffraction
3Å resolution

Structural insight into the membrane insertion of tail-anchored proteins by Get3

Released:
DOI: 10.2210/pdb3a37/pdb
PDB entry 3a37 coloured by chain, front view.
PDB entry 3a37 coloured by chain, side view.
PDB entry 3a37 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insight into the membrane insertion of tail-anchored proteins by Get3.
Yamagata A, Mimura H, Sato Y, Yamashita M, Yoshikawa A, Fukai S
Genes Cells 15 29-41 (2010)
PMID: 20015340

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATPase GET3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 40.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 115.229Å b: 222.554Å c: 49.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.253 0.282
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
7 more

4 mentions without citation

Structures of Get3, Get4, and Get5 provide new models for TA membrane protein targeting.
Simpson et al. (2010)
3 more