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Crystal Structure of H61A mutant TTHA0252 from Thermus thermophilus HB8

X-ray diffraction
2.5Å resolution
PDB entry 3a4y coloured by chain, front view.
PDB entry 3a4y coloured by chain, side view.
PDB entry 3a4y coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Ribonuclease TTHA0252
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Multimeric state: monomeric
Accessible surface area: 18164.14 Å2
Buried surface area: 3002.93 Å2
Dissociation area: 94.93 Å2
Dissociation energy (ΔGdiss): -1.64 kcal/mol
Dissociation entropy (TΔSdiss): 2.76 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-178188
Assembly 2
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Assembly Name: Ribonuclease TTHA0252
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Multimeric state: monomeric
Accessible surface area: 17759.59 Å2
Buried surface area: 2649.42 Å2
Dissociation area: 113.54 Å2
Dissociation energy (ΔGdiss): -1.74 kcal/mol
Dissociation entropy (TΔSdiss): 2.76 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-178188
Assembly 3 (preferred)
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Assembly Name: Ribonuclease TTHA0252
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Multimeric state: monomeric
Accessible surface area: 17948.17 Å2
Buried surface area: 2289.29 Å2
Dissociation area: 101.35 Å2
Dissociation energy (ΔGdiss): -2.62 kcal/mol
Dissociation entropy (TΔSdiss): 2.76 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-178188
Assembly 4
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Assembly Name: Ribonuclease TTHA0252
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Multimeric state: monomeric
Accessible surface area: 17783.79 Å2
Buried surface area: 1651.62 Å2
Dissociation area: 53.18 Å2
Dissociation energy (ΔGdiss): 6.58 kcal/mol
Dissociation entropy (TΔSdiss): 0.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-178188

Macromolecules

Ribonuclease TTHA0252
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.05 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLP1 (Residues: 1-431; Coverage: 100%)
Gene name: TTHA0252
Pfam:
InterPro:
CATH:
Ribonuclease TTHA0252 in PDB entry 3a4y, assembly 3, front view.
Ribonuclease TTHA0252 in PDB entry 3a4y, assembly 3, side view.
Ribonuclease TTHA0252 in PDB entry 3a4y, assembly 3, top view.
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