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Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II

X-ray diffraction
2Å resolution
PDB entry 3bz7 coloured by chain, front view.
PDB entry 3bz7 coloured by chain, side view.
PDB entry 3bz7 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Myosin-2 heavy chain
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Multimeric state: monomeric
Accessible surface area: 30746.21 Å2
Buried surface area: 1255.2 Å2
Dissociation area: 165.79 Å2
Dissociation energy (ΔGdiss): 5.72 kcal/mol
Dissociation entropy (TΔSdiss): 1.1 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140379
Assembly 2
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Assembly Name: Myosin-2 heavy chain
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Multimeric state: homo dimer
Accessible surface area: 59827.91 Å2
Buried surface area: 4201.22 Å2
Dissociation area: 887.19 Å2
Dissociation energy (ΔGdiss): -1.71 kcal/mol
Dissociation entropy (TΔSdiss): 15.3 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-530899

Macromolecules

Myosin-2 heavy chain
Chain: A
Length: 762 amino acids
Theoretical weight: 86.75 KDa
Source organism: Dictyostelium discoideum
Expression system: Not provided
UniProt:
  • Canonical: P08799 (Residues: 2-759; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Pfam:
InterPro:
SCOP: Myosin S1 fragment, N-terminal domain
Myosin-2 heavy chain in PDB entry 3bz7, assembly 1, front view.
Myosin-2 heavy chain in PDB entry 3bz7, assembly 1, side view.
Myosin-2 heavy chain in PDB entry 3bz7, assembly 1, top view.
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