› 3f7q

X-ray diffraction
1.75Å resolution

First pair of Fibronectin type III domains and part of the connecting segment of the integrin beta4

Released:
DOI: 10.2210/pdb3f7q/pdb
PDB entry 3f7q coloured by chain, front view.
PDB entry 3f7q coloured by chain, side view.
PDB entry 3f7q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the interaction between integrin alpha6beta4 and plectin at the hemidesmosomes.
de Pereda JM, Lillo MP, Sonnenberg A
EMBO J 28 1180-90 (2009)
PMID: 19242489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-538226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrin beta-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 26.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16144 (Residues: 1126-1355; Coverage: 13%)
Gene name: ITGB4
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

5 bound ligands:
Ligand CL 2 x CL
Ligand PEG 2 x PEG
Ligand 1PE 1 x 1PE
Ligand EDO 3 x EDO
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P1
Unit cell:
a: 43.11Å b: 49.47Å c: 74.36Å
α: 107.22° β: 96.06° γ: 106.6°
R-values:
R R work R free
0.157 0.155 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Type VII collagen: the anchoring fibril protein at fault in dystrophic epidermolysis bullosa.
Chung et al. (2010)
17 more

3 mentions without citation

Structural basis of the interaction between integrin alpha6beta4 and plectin at the hemidesmosomes.
de Pereda et al. (2009)
2 more