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DNA binding domain:GilZ 16bp complex-5

X-ray diffraction
1.9Å resolution
PDB entry 3g8u coloured by chain, front view.
PDB entry 3g8u coloured by chain, side view.
PDB entry 3g8u coloured by chain, top view.
Source organism: Rattus norvegicus
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glucocorticoid receptor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 12370.84 Å2
Buried surface area: 5115.66 Å2
Dissociation area: 1,287.28 Å2
Dissociation energy (ΔGdiss): 5.26 kcal/mol
Dissociation entropy (TΔSdiss): 11.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-116279

Macromolecules

Glucocorticoid receptor
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.96 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 440-525; Coverage: 11%)
Gene names: Grl, Nr3c1
Pfam: Zinc finger, C4 type (two domains)
InterPro:
CATH: Erythroid Transcription Factor GATA-1, subunit A
Glucocorticoid receptor in PDB entry 3g8u, assembly 1, front view.
Glucocorticoid receptor in PDB entry 3g8u, assembly 1, side view.
Glucocorticoid receptor in PDB entry 3g8u, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P06536  
190102030405060708090
 
20406080GSHMCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATAG
UniProt
P06536
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Interaction interfaces
Molecule details ›

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DNA chain D
Name: DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3')
Representative chains: D
Source organism: Rattus norvegicus [10116]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3') in PDB entry 3g8u, assembly 1, front view.
DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3') in PDB entry 3g8u, assembly 1, side view.
DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3') in PDB entry 3g8u, assembly 1, top view.
116246810121416
 
51015TGGAACCCAATGTTCT
Chains
RSRZ Outlier Chain C (auth D)
Chain C (auth D)
Interaction interfaces
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DNA chain C
Name: DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3')
Representative chains: C
Source organism: Rattus norvegicus [10116]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3') in PDB entry 3g8u, assembly 1, front view.
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3') in PDB entry 3g8u, assembly 1, side view.
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3') in PDB entry 3g8u, assembly 1, top view.
116246810121416
 
51015AAGAACATTGGGTTCC
Chains
Chain D (auth C)
Interaction interfaces
Molecule details ›

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