› 3hio

X-ray diffraction
2Å resolution

Crystal structure of Ricin A-chain in complex with the cyclic tetranucleotide inhibitor, a transition state analogue

Released:
DOI: 10.2210/pdb3hio/pdb
PDB entry 3hio coloured by chain, front view.
PDB entry 3hio coloured by chain, side view.
PDB entry 3hio coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins.
Ho MC, Sturm MB, Almo SC, Schramm VL
Proc Natl Acad Sci U S A 106 20276-81 (2009)
PMID: 19920175

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-527014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.07 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand C2X 1 x C2X
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 68.632Å b: 68.632Å c: 134.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.254
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Enzymatic transition states, transition-state analogs, dynamics, thermodynamics, and lifetimes.
Schramm VL. (2011)
12 more

9 mentions without citation

Ribosome-inactivating proteins: potent poisons and molecular tools.
Walsh et al. (2013)
8 more