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Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA

X-ray diffraction
2.6Å resolution
PDB entry 3hqg coloured by chain, front view.
PDB entry 3hqg coloured by chain, side view.
PDB entry 3hqg coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type II restriction enzyme EcoRII and DNA
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Multimeric state: hetero hexamer
PDBe Complex ID: PDB-CPX-116375

Macromolecules

Type II restriction enzyme EcoRII
Chain: A
Length: 222 amino acids
Theoretical weight: 25.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P14633 (Residues: 183-404; Coverage: 55%)
Gene name: ecoRIIR
Pfam: EcoRII C terminal
InterPro:
CATH: Restriction Endonuclease
Type II restriction enzyme EcoRII in PDB entry 3hqg, assembly 1, front view.
Type II restriction enzyme EcoRII in PDB entry 3hqg, assembly 1, side view.
Type II restriction enzyme EcoRII in PDB entry 3hqg, assembly 1, top view.
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DNA chain B
Name: 5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3'
Representative chains: B
Source organism: Escherichia coli [562]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3' in PDB entry 3hqg, assembly 1, front view.
5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3' in PDB entry 3hqg, assembly 1, side view.
5'-D(*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*A)-3' in PDB entry 3hqg, assembly 1, top view.
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DNA chain C
Name: 5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3'
Representative chains: C
Source organism: Escherichia coli [562]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3' in PDB entry 3hqg, assembly 1, front view.
5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3' in PDB entry 3hqg, assembly 1, side view.
5'-D(*TP*AP*GP*CP*CP*TP*GP*GP*TP*CP*GP*A)-3' in PDB entry 3hqg, assembly 1, top view.
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