› 3i5b

X-ray diffraction
2.04Å resolution

Crystal structure of the isolated GGDEF domain of WpsR from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3i5b/pdb
PDB entry 3i5b coloured by chain, front view.
PDB entry 3i5b coloured by chain, side view.
PDB entry 3i5b coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Determinants for the activation and autoinhibition of the diguanylate cyclase response regulator WspR.
De N, Navarro MV, Raghavan RV, Sondermann H
J Mol Biol 393 619-33 (2009)
PMID: 19695263

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 19.5 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXT9 (Residues: 172-347; Coverage: 51%)
Gene names: PA3702, wspR
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand TLA 2 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 35.615Å b: 94.497Å c: 97.592Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.193 0.244
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
21 more

5 mentions without citation

Sensing the messenger: the diverse ways that bacteria signal through c-di-GMP.
Krasteva et al. (2012)
4 more