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tmRNA-SmpB: a journey to the centre of the bacterial ribosome.

Weis F, Bron P, Giudice E, Rolland JP, Thomas D, Felden B, Gillet R
EMBO J 29 3810-8 (2010)
Cited: 38 times
EuropePMC logo PMID: 20953161

Abstract

Ribosomes mediate protein synthesis by decoding the information carried by messenger RNAs (mRNAs) and catalysing peptide bond formation between amino acids. When bacterial ribosomes stall on incomplete messages, the trans-translation quality control mechanism is activated by the transfer-messenger RNA bound to small protein B (tmRNA-SmpB ribonucleoprotein complex). Trans-translation liberates the stalled ribosomes and triggers degradation of the incomplete proteins. Here, we present the cryo-electron microscopy structures of tmRNA-SmpB accommodated or translocated into stalled ribosomes. Two atomic models for each state are proposed. This study reveals how tmRNA-SmpB crosses the ribosome and how, as the problematic mRNA is ejected, the tmRNA resume codon is placed onto the ribosomal decoding site by new contacts between SmpB and the nucleotides upstream of the tag-encoding sequence. This provides a structural basis for the transit of the large tmRNA-SmpB complex through the ribosome and for the means by which the tmRNA internal frame is set for translation to resume.

Reviews - 3iyq mentioned but not cited (2)

  1. Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB. Giudice E, Macé K, Gillet R. Front Microbiol 5 113 (2014)
  2. The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches. Gerovac M, Vogel J, Smirnov A. Front Mol Biosci 8 661448 (2021)

Articles - 3iyq mentioned but not cited (2)



Reviews citing this publication (15)

  1. Mechanisms of ribosome rescue in bacteria. Keiler KC. Nat. Rev. Microbiol. 13 285-297 (2015)
  2. The tmRNA ribosome-rescue system. Janssen BD, Hayes CS. Adv Protein Chem Struct Biol 86 151-191 (2012)
  3. Quality control systems for aberrant mRNAs induced by aberrant translation elongation and termination. Inada T. Biochim. Biophys. Acta 1829 634-642 (2013)
  4. The task force that rescues stalled ribosomes in bacteria. Giudice E, Gillet R. Trends Biochem. Sci. 38 403-411 (2013)
  5. RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview. Šponer J, Bussi G, Krepl M, Banáš P, Bottaro S, Cunha RA, Gil-Ley A, Pinamonti G, Poblete S, Jurečka P, Walter NG, Otyepka M. Chem. Rev. 118 4177-4338 (2018)
  6. tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell. Himeno H, Kurita D, Muto A. Front Genet 5 66 (2014)
  7. Ribosome-based quality control of mRNA and nascent peptides. Simms CL, Thomas EN, Zaher HS. Wiley Interdiscip Rev RNA 8 (2017)
  8. Ribosome rescue systems in bacteria. Himeno H, Nameki N, Kurita D, Muto A, Abo T. Biochimie 114 102-112 (2015)
  9. Ribosome Rescue Pathways in Bacteria. Müller C, Crowe-McAuliffe C, Wilson DN. Front Microbiol 12 652980 (2021)
  10. Structural Basis for Ribosome Rescue in Bacteria. Huter P, Müller C, Arenz S, Beckert B, Wilson DN. Trends Biochem. Sci. 42 669-680 (2017)
  11. Biochemical aspects of bacterial strategies for handling the incomplete translation processes. Shimizu Y. Front Microbiol 5 170 (2014)
  12. tRNA-mimicry in IRES-mediated translation and recoding. Butcher SE, Jan E. RNA Biol 13 1068-1074 (2016)
  13. The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes. Miller MR, Buskirk AR. Front Microbiol 5 462 (2014)
  14. [Protein synthesis by the ribosome: a pathway full of pitfalls]. Macé K, Giudice E, Gillet R. Med Sci (Paris) 31 282-290 (2015)
  15. Mechanism of trans-translation revealed by in vitro studies. Himeno H, Kurita D, Muto A. Front Microbiol 5 65 (2014)

Articles citing this publication (19)



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