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tmRNA-SmpB: a journey to the center of the bacterial ribosome

Electron Microscopy
13Å resolution
PDB entry 3iyq coloured by chain, front view.
PDB entry 3iyq coloured by chain, side view.
PDB entry 3iyq coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: SsrA-binding protein and RNA
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Multimeric state: hetero dimer
Accessible surface area: 61273.2 Å2
Buried surface area: 3906.25 Å2
Dissociation area: 1,953.12 Å2
Dissociation energy (ΔGdiss): 38.3 kcal/mol
Dissociation entropy (TΔSdiss): 12.87 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-290173

Macromolecules

SsrA-binding protein
Chain: B
Length: 149 amino acids
Theoretical weight: 17.48 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RR57 (Residues: 2-144; Coverage: 99%)
Gene names: TTHA0902, smpB
Pfam: SmpB protein
InterPro:
SsrA-binding protein in PDB entry 3iyq, assembly 1, front view.
SsrA-binding protein in PDB entry 3iyq, assembly 1, side view.
SsrA-binding protein in PDB entry 3iyq, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q8RR57  
114920406080100120140
 
50100150
UniProt
Q8RR57
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Sequence conservation
Molecule details ›

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RNA chain A
Name: tmRNA
Representative chains: A
Source organism: Thermus thermophilus HB8 [300852]
Length: 349 nucleotides
Theoretical weight: 112.82 KDa
Rfam: transfer-messenger RNA
tmRNA in PDB entry 3iyq, assembly 1, front view.
tmRNA in PDB entry 3iyq, assembly 1, side view.
tmRNA in PDB entry 3iyq, assembly 1, top view.
134950100150200250300
 
100200300
Chains
Chain A (auth A)
Rfam
Interaction interfaces
Molecule details ›

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