› 3jwe

X-ray diffraction
2.7Å resolution

Crystal structure of human mono-glyceride lipase in complex with SAR629

Released:
DOI: 10.2210/pdb3jwe/pdb
PDB entry 3jwe coloured by chain, front view.
PDB entry 3jwe coloured by chain, side view.
PDB entry 3jwe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for human monoglyceride lipase inhibition.
Bertrand T, Augé F, Houtmann J, Rak A, Vallée F, Mikol V, Berne PF, Michot N, Cheuret D, Hoornaert C, Mathieu M
J Mol Biol 396 663-73 (2010)
PMID: 19962385

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoglyceride lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 35.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99685 (Residues: 1-303; Coverage: 100%)
Gene name: MGLL
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand F4P 3 x F4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I222
Unit cell:
a: 87.821Å b: 138.506Å c: 127.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.224
Expression system: Escherichia coli

Quick links

Citations

16 review citations

FAT SIGNALS--lipases and lipolysis in lipid metabolism and signaling.
Zechner et al. (2012)
15 more

9 mentions without citation

Monoacylglycerol lipase inhibitors: modulators for lipid metabolism in cancer malignancy, neurological and metabolic disorders.
Deng et al. (2020)
8 more