› 3keo

X-ray diffraction
1.5Å resolution

Crystal Structure of a Rex-family transcriptional regulatory protein from Streptococcus agalactiae complexed with NAD+

Released:
DOI: 10.2210/pdb3keo/pdb
PDB entry 3keo coloured by chain, front view.
PDB entry 3keo coloured by chain, side view.
PDB entry 3keo coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup III
Primary publication:
NAD+ pool depletion as a signal for the Rex regulon involved in Streptococcus agalactiae virulence.
Franza T, Rogstam A, Thiyagarajan S, Sullivan MJ, Derré-Bobillot A, Bauer MC, Goh KGK, Da Cunha V, Glaser P, Logan DT, Ulett GC, von Wachenfeldt C, Gaudu P
OpenAccess logo PLoS Pathog 17 e1009791 (2021)
PMID: 34370789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Redox-sensing transcriptional repressor Rex Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.85 KDa
Source organism: Streptococcus agalactiae serogroup III
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E565 (Residues: 1-212; Coverage: 100%)
Gene names: gbs1167, rex
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAD 2 x NAD
Ligand MG 7 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21
Unit cell:
a: 46.27Å b: 92.41Å c: 47.19Å
α: 90° β: 91.26° γ: 90°
R-values:
R R work R free
0.178 0.175 0.234
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Butyrate enhances Clostridioides difficile sporulation in vitro.
Baldassare et al. (2023)
1 more

3 mentions without citation

Validation of metal-binding sites in macromolecular structures with the CheckMyMetal web server.
Zheng et al. (2014)
2 more