› 3mkl

X-ray diffraction
2.15Å resolution

Crystal structure of DNA-binding transcriptional dual regulator from Escherichia coli K-12

Released:
DOI: 10.2210/pdb3mkl/pdb
PDB entry 3mkl coloured by chain, front view.
PDB entry 3mkl coloured by chain, side view.
PDB entry 3mkl coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Chang C, Mack J, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator GadX Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 14.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37639 (Residues: 137-255; Coverage: 43%)
Gene names: JW3484, b3516, gadX, yhiX
Sequence domains: Bacterial regulatory helix-turn-helix proteins, AraC family
Structure domains: Homeodomain-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 41.972Å b: 81.856Å c: 165.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.247
Expression system: Escherichia coli BL21(DE3)

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