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Native structure of a c-di-GMP riboswitch from V. cholerae

X-ray diffraction
2.3Å resolution
PDB entry 3mxh coloured by chain, front view.
PDB entry 3mxh coloured by chain, side view.
PDB entry 3mxh coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: U1 small nuclear ribonucleoprotein A and RNA
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Multimeric state: hetero dimer
Accessible surface area: 19246.1 Å2
Buried surface area: 3814.11 Å2
Dissociation area: 987.96 Å2
Dissociation energy (ΔGdiss): 5.57 kcal/mol
Dissociation entropy (TΔSdiss): 11.4 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140493

Macromolecules

U1 small nuclear ribonucleoprotein A
Chain: P
Length: 98 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Pfam: RNA recognition motif
InterPro:
CATH: Alpha-Beta Plaits
U1 small nuclear ribonucleoprotein A in PDB entry 3mxh, assembly 1, front view.
U1 small nuclear ribonucleoprotein A in PDB entry 3mxh, assembly 1, side view.
U1 small nuclear ribonucleoprotein A in PDB entry 3mxh, assembly 1, top view.
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RNA chain R
Name: c-di-GMP riboswitch
Representative chains: R
Source organism: Vibrio cholerae [666]
Expression system: Not provided
Length: 92 nucleotides
Theoretical weight: 29.94 KDa
Rfam: Cyclic di-GMP-I riboswitch
c-di-GMP riboswitch in PDB entry 3mxh, assembly 1, front view.
c-di-GMP riboswitch in PDB entry 3mxh, assembly 1, side view.
c-di-GMP riboswitch in PDB entry 3mxh, assembly 1, top view.
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