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TUBULIN-NSC 613863: RB3 Stathmin-like domain complex

X-ray diffraction
4Å resolution
PDB entry 3n2g coloured by chain, front view.
PDB entry 3n2g coloured by chain, side view.
PDB entry 3n2g coloured by chain, top view.
Source organisms:
Assembly composition:
hetero pentamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 65973.3 Å2
Buried surface area: 17307.21 Å2
Dissociation area: 2,722.05 Å2
Dissociation energy (ΔGdiss): 24.61 kcal/mol
Dissociation entropy (TΔSdiss): 15.16 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-112169

Macromolecules

Tubulin alpha chain
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.19 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Pfam:
InterPro:
CATH:
Tubulin alpha chain in PDB entry 3n2g, assembly 1, front view.
Tubulin alpha chain in PDB entry 3n2g, assembly 1, side view.
Tubulin alpha chain in PDB entry 3n2g, assembly 1, top view.
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Tubulin beta chain
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Pfam:
InterPro:
CATH:
Tubulin beta chain in PDB entry 3n2g, assembly 1, front view.
Tubulin beta chain in PDB entry 3n2g, assembly 1, side view.
Tubulin beta chain in PDB entry 3n2g, assembly 1, top view.
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Stathmin-4
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Pfam: Stathmin family
InterPro:
CATH: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Stathmin-4 in PDB entry 3n2g, assembly 1, front view.
Stathmin-4 in PDB entry 3n2g, assembly 1, side view.
Stathmin-4 in PDB entry 3n2g, assembly 1, top view.
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