› 3n4s

X-ray diffraction
2.35Å resolution

Structure of Csm1 C-terminal domain, P21212 form

Released:
DOI: 10.2210/pdb3n4s/pdb
PDB entry 3n4s coloured by chain, front view.
PDB entry 3n4s coloured by chain, side view.
PDB entry 3n4s coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments.
Corbett KD, Yip CK, Ee LS, Walz T, Amon A, Harrison SC
Cell 142 556-67 (2010)
PMID: 20723757

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monopolin complex subunit CSM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 116 amino acids
Theoretical weight: 13.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25651 (Residues: 69-181; Coverage: 60%)
Gene names: CSM1, SPO86, YCR086W, YCR86W
Sequence domains: Chromosome segregation protein Csm1/Pcs1
Structure domains: Aspartate Aminotransferase, domain 1

Ligands and Environments

1 bound ligand:
Ligand 1PE 10 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 69.975Å b: 134.044Å c: 54.419Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Causes and consequences of aneuploidy in cancer.
Gordon et al. (2012)
26 more

3 mentions without citation

The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments.
Corbett et al. (2010)
2 more