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yeast Ent3_ENTH-Vti1p_Habc complex structure

X-ray diffraction
2.2Å resolution
PDB entry 3onl coloured by chain, front view.
PDB entry 3onl coloured by chain, side view.
PDB entry 3onl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero trimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Epsin-3 and t-SNARE VTI1
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Multimeric state: hetero trimer
Accessible surface area: 18963.87 Å2
Buried surface area: 2687.03 Å2
Dissociation area: 1,343.51 Å2
Dissociation energy (ΔGdiss): -11.92 kcal/mol
Dissociation entropy (TΔSdiss): 22.89 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155607

Macromolecules

Epsin-3
Chains: A, B
Length: 150 amino acids
Theoretical weight: 17.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47160 (Residues: 28-170; Coverage: 35%)
Gene names: ENT3, J2048, YJR125C
Pfam: ENTH domain
InterPro:
CATH: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Epsin-3 in PDB entry 3onl, assembly 1, front view.
Epsin-3 in PDB entry 3onl, assembly 1, side view.
Epsin-3 in PDB entry 3onl, assembly 1, top view.
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t-SNARE VTI1
Chain: C
Length: 97 amino acids
Theoretical weight: 11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04338 (Residues: 3-99; Coverage: 45%)
Gene names: VTI1, YM9646.10C, YMR197C
Pfam: Vesicle transport v-SNARE protein N-terminus
InterPro:
CATH: t-snare proteins
t-SNARE VTI1 in PDB entry 3onl, assembly 1, front view.
t-SNARE VTI1 in PDB entry 3onl, assembly 1, side view.
t-SNARE VTI1 in PDB entry 3onl, assembly 1, top view.
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