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Urate oxidase under 1 MPa / 10 bars pressure of nitrous oxide

X-ray diffraction
1.75Å resolution
PDB entry 3pku coloured by chain, front view.
PDB entry 3pku coloured by chain, side view.
PDB entry 3pku coloured by chain, top view.
Source organism: Aspergillus flavus
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Uricase
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Multimeric state: homo tetramer
Accessible surface area: 44146.18 Å2
Buried surface area: 27157.91 Å2
Dissociation area: 5,817.92 Å2
Dissociation energy (ΔGdiss): 82.48 kcal/mol
Dissociation entropy (TΔSdiss): 16.11 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-169432

Macromolecules

Uricase
Chain: A
Length: 302 amino acids
Theoretical weight: 34.18 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Pfam: Uricase
InterPro:
CATH: Urate Oxidase;
Uricase in PDB entry 3pku, assembly 1, front view.
Uricase in PDB entry 3pku, assembly 1, side view.
Uricase in PDB entry 3pku, assembly 1, top view.
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