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Structure of Hub-1 protein in complex with Snu66 peptide (HINDI)

X-ray diffraction
1.4Å resolution
PDB entry 3plu coloured by chain, front view.
PDB entry 3plu coloured by chain, side view.
PDB entry 3plu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component and Ubiquitin-like modifier HUB1
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Multimeric state: hetero dimer
Accessible surface area: 5339.83 Å2
Buried surface area: 997.27 Å2
Dissociation area: 498.64 Å2
Dissociation energy (ΔGdiss): 3.68 kcal/mol
Dissociation entropy (TΔSdiss): 7.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-171358
Assembly 2
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Assembly Name: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component and Ubiquitin-like modifier HUB1
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Multimeric state: hetero dimer
Accessible surface area: 5346.88 Å2
Buried surface area: 991.48 Å2
Dissociation area: 495.74 Å2
Dissociation energy (ΔGdiss): 3.39 kcal/mol
Dissociation entropy (TΔSdiss): 7.87 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-171358

Macromolecules

Ubiquitin-like modifier HUB1
Chains: A, B
Length: 93 amino acids
Theoretical weight: 10.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6Q546 (Residues: 1-73; Coverage: 100%)
Gene names: HUB1, YNR032C-A
Pfam: Ubiquitin family
InterPro:
CATH: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin-like modifier HUB1 in PDB entry 3plu, assembly 1, front view.
Ubiquitin-like modifier HUB1 in PDB entry 3plu, assembly 1, side view.
Ubiquitin-like modifier HUB1 in PDB entry 3plu, assembly 1, top view.
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66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Chains: C, D
Length: 19 amino acids
Theoretical weight: 2.22 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: Q12420 (Residues: 6-24; Coverage: 3%)
Gene names: 05667, SNU66, YOR308C
Pfam: HIND motif
InterPro: HIND motif
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component in PDB entry 3plu, assembly 1, front view.
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component in PDB entry 3plu, assembly 1, side view.
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component in PDB entry 3plu, assembly 1, top view.
Molecule details ›

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