› 3qd2

X-ray diffraction
2.81Å resolution

Crystal structure of mouse PERK kinase domain

Released:
DOI: 10.2210/pdb3qd2/pdb
PDB entry 3qd2 coloured by chain, front view.
PDB entry 3qd2 coloured by chain, side view.
PDB entry 3qd2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structure of the PERK kinase domain suggests the mechanism for its activation.
Cui W, Li J, Ron D, Sha B
Acta Crystallogr D Biol Crystallogr 67 423-8 (2011)
PMID: 21543844

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579136 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 2-alpha kinase 3 Chain: B
Molecule details ›
Chain: B
Length: 332 amino acids
Theoretical weight: 38.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Z2B5 (Residues: 582-1078; Coverage: 31%)
Gene names: Eif2ak3, Pek, Perk
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P41212
Unit cell:
a: 97.891Å b: 97.891Å c: 116.706Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.27 0.332
Expression system: Escherichia coli BL21

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Citations

36 review citations

Mechanisms, regulation and functions of the unfolded protein response.
Hetz et al. (2020)
35 more

9 mentions without citation

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Wu et al. (2014)
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