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Crystal structure of complex of 4PAL (4-Pyridoxolactone) and PLDH (tetrameric pyridoxal 4-dehydrogenase) from Mesorhizobium loti

X-ray diffraction
1.699Å resolution
PDB entry 3rwb coloured by chain, front view.
PDB entry 3rwb coloured by chain, side view.
PDB entry 3rwb coloured by chain, top view.
Source organism: Mesorhizobium loti
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Pyridoxal 4-dehydrogenase
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Multimeric state: homo tetramer
Accessible surface area: 28548.27 Å2
Buried surface area: 19832.69 Å2
Dissociation area: 3,991.21 Å2
Dissociation energy (ΔGdiss): 27.47 kcal/mol
Dissociation entropy (TΔSdiss): 14.9 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-189125

Macromolecules

Pyridoxal 4-dehydrogenase
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 25.43 KDa
Source organism: Mesorhizobium loti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q988B7 (Residues: 2-248; Coverage: 100%)
Gene names: mlr6807, pldh-t
Pfam: Enoyl-(Acyl carrier protein) reductase
InterPro:
CATH: NAD(P)-binding Rossmann-like Domain
Pyridoxal 4-dehydrogenase in PDB entry 3rwb, assembly 1, front view.
Pyridoxal 4-dehydrogenase in PDB entry 3rwb, assembly 1, side view.
Pyridoxal 4-dehydrogenase in PDB entry 3rwb, assembly 1, top view.
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