› 3s0x

X-ray diffraction
3.6Å resolution

The crystal structure of GxGD membrane protease FlaK

Released:
DOI: 10.2210/pdb3s0x/pdb
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Source organism: Methanococcus maripaludis
Primary publication:
The crystal structure of GXGD membrane protease FlaK.
Hu J, Xue Y, Lee S, Ha Y
Nature 475 528-31 (2011)
PMID: 21765428

Function and Biology Details

Reaction catalysed: 
Cleaves the signal peptide of 3 to 12 amino acids from the N-terminal of preflagellin, usually at Arg-Gly-|- or Lys-Gly-|-, to release flagellin.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Preflagellin peptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.08 KDa
Source organism: Methanococcus maripaludis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A9A677 (Residues: 1-230; Coverage: 100%)
Gene names: MmarC6_0338, flaK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 70.895Å b: 99.715Å c: 118.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.27 0.327
Expression system: Escherichia coli BL21

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Citations

18 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
17 more

10 mentions without citation

Membrane proteases in the bacterial protein secretion and quality control pathway.
Dalbey et al. (2012)
9 more

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