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MUTM Slanted complex 8 with R112A mutation

X-ray diffraction
2.35Å resolution
PDB entry 3saw coloured by chain, front view.
PDB entry 3saw coloured by chain, side view.
PDB entry 3saw coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Formamidopyrimidine-DNA glycosylase and DNA
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Multimeric state: hetero trimer
Accessible surface area: 13615.64 Å2
Buried surface area: 2958.78 Å2
Dissociation area: 1,014.22 Å2
Dissociation energy (ΔGdiss): 7.86 kcal/mol
Dissociation entropy (TΔSdiss): 11 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-116873

Macromolecules

Formamidopyrimidine-DNA glycosylase
Chain: A
Length: 273 amino acids
Theoretical weight: 30.5 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84131 (Residues: 2-274; Coverage: 100%)
Gene names: fpg, mutM
Pfam:
InterPro:
CATH:
Formamidopyrimidine-DNA glycosylase in PDB entry 3saw, assembly 1, front view.
Formamidopyrimidine-DNA glycosylase in PDB entry 3saw, assembly 1, side view.
Formamidopyrimidine-DNA glycosylase in PDB entry 3saw, assembly 1, top view.
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DNA chain B
Name: 5'-D(*A*GP*GP*TP*AP*GP*AP*CP*AP*AP*GP*GP*AP*CP*GP*C)-3'
Representative chains: B
Source organism: Geobacillus stearothermophilus [1422]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.98 KDa
5'-D(*A*GP*GP*TP*AP*GP*AP*CP*AP*AP*GP*GP*AP*CP*GP*C)-3' in PDB entry 3saw, assembly 1, front view.
5'-D(*A*GP*GP*TP*AP*GP*AP*CP*AP*AP*GP*GP*AP*CP*GP*C)-3' in PDB entry 3saw, assembly 1, side view.
5'-D(*A*GP*GP*TP*AP*GP*AP*CP*AP*AP*GP*GP*AP*CP*GP*C)-3' in PDB entry 3saw, assembly 1, top view.
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DNA chain C
Name: 5'-D(*TP*GP*CP*GP*TP*C*CP*TP*TP*GP*TP*(CX2)P*TP*AP*CP*C)-3'
Representative chains: C
Source organism: Geobacillus stearothermophilus [1422]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
5'-D(*TP*GP*CP*GP*TP*C*CP*TP*TP*GP*TP*(CX2)P*TP*AP*CP*C)-3' in PDB entry 3saw, assembly 1, front view.
5'-D(*TP*GP*CP*GP*TP*C*CP*TP*TP*GP*TP*(CX2)P*TP*AP*CP*C)-3' in PDB entry 3saw, assembly 1, side view.
5'-D(*TP*GP*CP*GP*TP*C*CP*TP*TP*GP*TP*(CX2)P*TP*AP*CP*C)-3' in PDB entry 3saw, assembly 1, top view.
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