› › Structure analysis

Inward facing conformations of the MetNI methionine ABC transporter: CY5 SeMet soak crystal form

X-ray diffraction
3.4Å resolution
PDB entry 3tuz coloured by chain, front view.
PDB entry 3tuz coloured by chain, side view.
PDB entry 3tuz coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: Methionine import ATP-binding protein MetN and D-methionine transport system permease protein MetI
hide full assembly name
Multimeric state: hetero tetramer
Accessible surface area: 49920.84 Å2
Buried surface area: 10208.36 Å2
Dissociation area: 2,270.08 Å2
Dissociation energy (ΔGdiss): 13 kcal/mol
Dissociation entropy (TΔSdiss): 14.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-151799
Assembly 2
Download    3D Visualisation
Assembly Name: Methionine import ATP-binding protein MetN and D-methionine transport system permease protein MetI
hide full assembly name
Multimeric state: hetero tetramer
Accessible surface area: 49549.05 Å2
Buried surface area: 10066.75 Å2
Dissociation area: 1,697.8 Å2
Dissociation energy (ΔGdiss): 11.49 kcal/mol
Dissociation entropy (TΔSdiss): 14.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-151799

Macromolecules

D-methionine transport system permease protein MetI
Chains: A, B, E, F
Length: 217 amino acids
Theoretical weight: 23.27 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31547 (Residues: 1-217; Coverage: 100%)
Gene names: JW0194, b0198, metI, yaeE
Pfam: Binding-protein-dependent transport system inner membrane component
InterPro:
CATH: MetI-like
D-methionine transport system permease protein MetI in PDB entry 3tuz, assembly 1, front view.
D-methionine transport system permease protein MetI in PDB entry 3tuz, assembly 1, side view.
D-methionine transport system permease protein MetI in PDB entry 3tuz, assembly 1, top view.
Molecule details ›

Search similar proteins

Methionine import ATP-binding protein MetN
Chains: C, D, G, H
Length: 366 amino acids
Theoretical weight: 40.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P30750 (Residues: 1-343; Coverage: 100%)
Gene names: JW0195, abc, b0199, metN
Pfam:
InterPro:
CATH:
Methionine import ATP-binding protein MetN in PDB entry 3tuz, assembly 1, front view.
Methionine import ATP-binding protein MetN in PDB entry 3tuz, assembly 1, side view.
Methionine import ATP-binding protein MetN in PDB entry 3tuz, assembly 1, top view.
Molecule details ›

Search similar proteins

Quick links