› 3tw7

X-ray diffraction
3.1Å resolution

Structure of Rhizobium etli pyruvate carboxylase T882A crystallized without acetyl coenzyme-A

Released:
DOI: 10.2210/pdb3tw7/pdb
PDB entry 3tw7 coloured by chain, front view.
PDB entry 3tw7 coloured by chain, side view.
PDB entry 3tw7 coloured by chain, top view.
Source organism: Rhizobium etli CFN 42
Primary publication:
Interaction between the biotin carboxyl carrier domain and the biotin carboxylase domain in pyruvate carboxylase from Rhizobium etli.
Lietzan AD, Menefee AL, Zeczycki TN, Kumar S, Attwood PV, Wallace JC, Cleland WW, St Maurice M
Biochemistry 50 9708-23 (2011)
PMID: 21958016

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate carboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1165 amino acids
Theoretical weight: 127.53 KDa
Source organism: Rhizobium etli CFN 42
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2K340 (Residues: 2-1154; Coverage: 100%)
Gene names: RHE_CH04002, pyc
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ZN 2 x ZN
Ligand CL 2 x CL
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I41
Unit cell:
a: 264.158Å b: 264.158Å c: 91.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.292
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structure and function of biotin-dependent carboxylases.
Tong L. (2013)
4 more

2 mentions without citation

Interaction between the biotin carboxyl carrier domain and the biotin carboxylase domain in pyruvate carboxylase from Rhizobium etli.
Lietzan et al. (2011)
1 more