› 3uhj

X-ray diffraction
2.34Å resolution

Crystal structure of a probable glycerol dehydrogenase from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb3uhj/pdb
PDB entry 3uhj coloured by chain, front view.
PDB entry 3uhj coloured by chain, side view.
PDB entry 3uhj coloured by chain, top view.
Source organism: Sinorhizobium meliloti
Entry authors: Agarwal R, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
Glycerol + NAD(+) = glycerone + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-187888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 387 amino acids
Theoretical weight: 41.57 KDa
Source organism: Sinorhizobium meliloti
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92MR2 (Residues: 1-364; Coverage: 100%)
Gene names: SMc02038, gldA
Sequence domains: Iron-containing alcohol dehydrogenase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 6 x GOL
Ligand ZN 8 x ZN
Ligand SE 2 x SE
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 79.483Å b: 98.996Å c: 104.734Å
α: 76.94° β: 83.38° γ: 71.47°
R-values:
R R work R free
0.2 0.197 0.264
Expression system: Escherichia coli BL21(DE3)

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