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Crystal structure of a RNA-binding domain of a poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 1.38 A resolution

X-ray diffraction
1.38Å resolution
PDB entry 3us5 coloured by chain, front view.
PDB entry 3us5 coloured by chain, side view.
PDB entry 3us5 coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Poly(U)-binding-splicing factor PUF60
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Multimeric state: monomeric
Accessible surface area: 7948.87 Å2
Buried surface area: 113.74 Å2
Dissociation area: 56.87 Å2
Dissociation energy (ΔGdiss): 7.28 kcal/mol
Dissociation entropy (TΔSdiss): -0.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193769

Macromolecules

Poly(U)-binding-splicing factor PUF60
Chain: A
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHX1 (Residues: 443-559; Coverage: 21%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
InterPro:
CATH: Alpha-Beta Plaits
Poly(U)-binding-splicing factor PUF60 in PDB entry 3us5, assembly 1, front view.
Poly(U)-binding-splicing factor PUF60 in PDB entry 3us5, assembly 1, side view.
Poly(U)-binding-splicing factor PUF60 in PDB entry 3us5, assembly 1, top view.
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