Function and Biology Details
Reaction catalysed:
Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Effector protein NleF and Caspase-9 subunit p35 (preferred)
PDBe Complex ID:
PDB-CPX-157265 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-9 subunit p35
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 276 amino acids
Theoretical weight: 30.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Length: 276 amino acids
Theoretical weight: 30.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P55211 (Residues: 141-416; Coverage: 66%)
Sequence domains: Caspase domain
Structure domains: Rossmann fold
Effector protein NleF
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 165 amino acids
Theoretical weight: 18.87 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
Sequence domains: NleF caspase inhibitor
Structure domains: Ferritin
Length: 165 amino acids
Theoretical weight: 18.87 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
- Canonical: Q8XAL7 (Residues: 25-189; Coverage: 87%)
Sequence domains: NleF caspase inhibitor
Structure domains: Ferritin
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
SLS BEAMLINE X06SA
Spacegroup:
P212121
Expression system: Escherichia coli
