› 3v3l

X-ray diffraction
1.65Å resolution

Crystal structure of human RNF146 WWE domain in complex with iso-ADPRibose

Released:
DOI: 10.2210/pdb3v3l/pdb
PDB entry 3v3l coloured by chain, front view.
PDB entry 3v3l coloured by chain, side view.
PDB entry 3v3l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of the iso-ADP-ribose moiety in poly(ADP-ribose) by WWE domains suggests a general mechanism for poly(ADP-ribosyl)ation-dependent ubiquitination.
Wang Z, Michaud GA, Cheng Z, Zhang Y, Hinds TR, Fan E, Cong F, Xu W
Genes Dev 26 235-40 (2012)
PMID: 22267412

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase RNF146 Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NTX7 (Residues: 100-184; Coverage: 24%)
Gene name: RNF146
Sequence domains: WWE domain
Structure domains: Signal recognition particle alu RNA binding heterodimer, srp9/1

Ligands and Environments

1 bound ligand:
Ligand V3L 2 x V3L
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3112
Unit cell:
a: 55.343Å b: 55.343Å c: 146.471Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.181
Expression system: Escherichia coli

Quick links

Citations

52 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
51 more

9 mentions without citation

PARPs and ADP-ribosylation: recent advances linking molecular functions to biological outcomes.
Gupte et al. (2017)
8 more