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Crystal structure of GYE (old yellow enzyme)

X-ray diffraction
3.3Å resolution
PDB entry 3wjs coloured by chain, front view.
PDB entry 3wjs coloured by chain, side view.
PDB entry 3wjs coloured by chain, top view.
Source organism: Gluconobacter oxydans
Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 12479.67 Å2
Buried surface area: 591.46 Å2
Dissociation area: 128.57 Å2
Dissociation energy (ΔGdiss): -5.1 kcal/mol
Dissociation entropy (TΔSdiss): 2.28 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106597
Assembly 2
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 22386.24 Å2
Buried surface area: 3756.62 Å2
Dissociation area: 1,286.55 Å2
Dissociation energy (ΔGdiss): 6.93 kcal/mol
Dissociation entropy (TΔSdiss): 13.52 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-106598

Macromolecules

NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
Chain: A
Length: 361 amino acids
Theoretical weight: 39.29 KDa
Source organism: Gluconobacter oxydans
Expression system: Escherichia coli
UniProt:
  • Canonical: A1E8I9 (Residues: 1-361; Coverage: 100%)
Gene names: AD934_01855, AD950_09540, EDC20_102158, nox
Pfam: NADH:flavin oxidoreductase / NADH oxidase family
InterPro:
CATH: Aldolase class I
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 3wjs, assembly 1, front view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 3wjs, assembly 1, side view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 3wjs, assembly 1, top view.
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