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Crystal structure of the Sgt2 N domain and the Get5 UBL domain complex

X-ray diffraction
1.803Å resolution
PDB entry 3zdm coloured by chain, front view.
PDB entry 3zdm coloured by chain, side view.
PDB entry 3zdm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero hexamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Ubiquitin-like protein MDY2 and Small glutamine-rich tetratricopeptide repeat-containing protein 2
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Multimeric state: hetero hexamer
Accessible surface area: 19516.14 Å2
Buried surface area: 7644.73 Å2
Dissociation area: 1,234.78 Å2
Dissociation energy (ΔGdiss): -2.68 kcal/mol
Dissociation entropy (TΔSdiss): 21.95 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-171299

Macromolecules

Small glutamine-rich tetratricopeptide repeat-containing protein 2
Chains: A, B, D, E
Length: 72 amino acids
Theoretical weight: 7.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12118 (Residues: 1-72; Coverage: 21%)
Gene names: SGT2, UNF346, YOR007C
Pfam: Homodimerisation domain of SGTA
InterPro: SGTA, homodimerisation domain
CATH: Immunoglobulin FC, subunit C
Small glutamine-rich tetratricopeptide repeat-containing protein 2 in PDB entry 3zdm, assembly 1, front view.
Small glutamine-rich tetratricopeptide repeat-containing protein 2 in PDB entry 3zdm, assembly 1, side view.
Small glutamine-rich tetratricopeptide repeat-containing protein 2 in PDB entry 3zdm, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q12118  
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Ubiquitin-like protein MDY2
Chains: C, F
Length: 81 amino acids
Theoretical weight: 9.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12285 (Residues: 71-151; Coverage: 38%)
Gene names: GET5, MDY2, TMA24, YOL111C
Pfam: Ubiquitin family
InterPro:
CATH: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin-like protein MDY2 in PDB entry 3zdm, assembly 1, front view.
Ubiquitin-like protein MDY2 in PDB entry 3zdm, assembly 1, side view.
Ubiquitin-like protein MDY2 in PDB entry 3zdm, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q12285  
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Molecule details ›

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