› 4bzf

X-ray diffraction
1.9Å resolution

Crystal structure of galactose mutarotase GalM from Bacillus subtilis with trehalose

Released:
DOI: 10.2210/pdb4bzf/pdb
PDB entry 4bzf coloured by chain, front view.
PDB entry 4bzf coloured by chain, side view.
PDB entry 4bzf coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Entry authors: Vanden Broeck A, Sauvage E, Herman R, Kerff F, Duez C, Charlier P

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose = beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-544345 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Aldose 1-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P39840 (Residues: 2-325; Coverage: 100%)
Gene names: BSU18360, galM, yoxA
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand PGE 1 x PGE
Ligand CIT 2 x CIT
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21212
Unit cell:
a: 82.19Å b: 83.322Å c: 124.446Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.238
Expression system: Escherichia coli K-12

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