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Crystal structure of galactose mutarotase GalM from Bacillus subtilis with trehalose

X-ray diffraction
1.9Å resolution
PDB entry 4bzf coloured by chain, front view.
PDB entry 4bzf coloured by chain, side view.
PDB entry 4bzf coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Aldose 1-epimerase
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Multimeric state: monomeric
Accessible surface area: 13390.69 Å2
Buried surface area: 1331.3 Å2
Dissociation area: 176.37 Å2
Dissociation energy (ΔGdiss): -5.2 kcal/mol
Dissociation entropy (TΔSdiss): 2.38 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-544345
Assembly 2
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Assembly Name: Aldose 1-epimerase
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Multimeric state: monomeric
Accessible surface area: 13898.46 Å2
Buried surface area: 932.22 Å2
Dissociation area: 49.23 Å2
Dissociation energy (ΔGdiss): -3.47 kcal/mol
Dissociation entropy (TΔSdiss): 2.77 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-544345

Macromolecules

Aldose 1-epimerase
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P39840 (Residues: 2-325; Coverage: 100%)
Gene names: BSU18360, galM, yoxA
Pfam: Aldose 1-epimerase
InterPro:
CATH: Beta-galactosidase; Chain A, domain 5
Aldose 1-epimerase in PDB entry 4bzf, assembly 1, front view.
Aldose 1-epimerase in PDB entry 4bzf, assembly 1, side view.
Aldose 1-epimerase in PDB entry 4bzf, assembly 1, top view.
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