› 4exm

X-ray diffraction
2.6Å resolution

The crystal structure of an engineered phage lysin containing the binding domain of pesticin and the killing domain of T4-lysozyme

Released:
DOI: 10.2210/pdb4exm/pdb
PDB entry 4exm coloured by chain, front view.
PDB entry 4exm coloured by chain, side view.
PDB entry 4exm coloured by chain, top view.
Source organisms:
Primary publication:
Structural engineering of a phage lysin that targets gram-negative pathogens.
Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK
Proc Natl Acad Sci U S A 109 9857-62 (2012)
PMID: 22679291

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Pesticin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 347 amino acids
Theoretical weight: 39.7 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q57159 (Residues: 1-167; Coverage: 47%)
  • Canonical: P00720 (Residues: 3-164; Coverage: 99%)
Gene names: E, YPPCP1.05c, pst
Sequence domains:
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P32
Unit cell:
a: 108.427Å b: 108.427Å c: 109.752Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.251
Expression system: Escherichia coli

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Citations

34 review citations

Iron, copper, zinc, and manganese transport and regulation in pathogenic Enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence.
Porcheron et al. (2013)
33 more