› 4b1r

X-ray diffraction
1.95Å resolution

Tetracycline repressor class D mutant H100A in complex with iso-7- Chlortetracycline

Released:
DOI: 10.2210/pdb4b1r/pdb
PDB entry 4b1r coloured by chain, front view.
PDB entry 4b1r coloured by chain, side view.
PDB entry 4b1r coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Eltschkner S, Palm GJ, Schneider J, Hinrichs W

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0ACT4 (Residues: 3-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ITC 1 x ITC
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: I4122
Unit cell:
a: 68.36Å b: 68.36Å c: 179.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.26
Expression system: Escherichia coli K-12

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